منابع مشابه
Nucleosome and transcription activator antagonism at human β-globin locus control region DNase I hypersensitive sites
Locus control regions are regulatory elements that activate distant genes and typically consist of several DNase I hypersensitive sites coincident with clusters of transcription activator binding sites. To what extent nucleosomes and activators occupy these sites together or exclusively has not been extensively studied in vivo. We analyzed the chromatin structure of human beta-globin locus cont...
متن کامل5′HS5 of the Human β-globin Locus Control Region Is Dispensable for the Formation of the β-globin Active Chromatin Hub
Hypersensitive site 5 (5'HS5) of the beta-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 5'HS5 in the three dimensional organization of the beta-gene locus using the Chromatin Conformation Capture (3C) technique. The results show that when 5'HS5 is deleted from the locus, both remote and internal regulatory e...
متن کاملStructural polymorphism exhibited by a quasipalindrome present in the locus control region (LCR) of the human β-globin gene cluster
Structural polymorphism of DNA is a widely accepted property. A simple addition to this perception has been our recent finding, where a single nucleotide polymorphism (SNP) site present in a quasipalindromic sequence of beta-globin LCR exhibited a hairpin-duplex equilibrium. Our current studies explore that secondary structures adopted by individual complementary strands compete with formation ...
متن کاملReconstitution of human beta-globin locus control region hypersensitive sites in the absence of chromatin assembly.
The human beta-globin genes are regulated by the locus control region (LCR), an element composed of multiple DNase I-hypersensitive sites (HS sites) located 5' to the genes. Various functional studies indicate that the LCR confers high-level, position-independent, and copy number-dependent expression to linked globin genes in transgenic mice. However, the structural basis for LCR function is un...
متن کاملRED CELLS Deletion of the core region of 5 HS2 of the mouse -globin locus control region reveals a distinct effect in comparison with human -globin transgenes
The -globin locus control region (LCR) is a large DNA element that is required for high-level expression of -like globin genes from the endogenous mouse locus or in transgenic mice carrying the human -globin locus. The LCR encompasses 6 DNaseI hypersensitive sites (HSs) that bind transcription factors. These HSs each contain a core of a few hundred base pairs (bp) that has most of the functiona...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2002
ISSN: 0014-2956
DOI: 10.1046/j.1432-1327.2002.02797.x